Optical trapping-controlled protein crystallization
Teruki Sugiyama1*
1Department of Applied Chemistry and Institute of Molecular Science, National Chiao Tung University, Hsinchu, Taiwan
* presenting author:Teruki Sugiyama, email:sugiyama@nctu.edu.tw
We have succeeded so far in demonstrating optical trapping-induced crystallization of various amino acids and in controlling their crystal polymorphism and growth [1-4]. Recently, we extended target compounds from amino acids to proteins, and successfully controlled crystallization of hen egg-white lysozyme (HEWL) utilizing optical trapping. Optical trapping of HEWL liquid-like clusters in solution firstly creates a highly concentrated domain consisting of the clusters locally at the laser focus, and the domain then propagated to outside of the focus through mutual intercluster interactions. HEWL nucleation and crystal growth are triggered in the large cluster domain and are controlled by concentration, ordering and anisotropy of the clusters in the domain depending on laser power and polarization. We imply that the mechanism involving such large highly concentrated domain of clusters should be general for various kinds of compounds with hydrogen bonds.

[1] T. Sugiyama, T. Adachi, and H. Masuhara, Chem. Lett., 36, 1480 (2007).
[2] T. Sugiyama, K. Yuyama, and H. Masuhara, Acc. Chem. Res., 45, 1946 (2012).
[3] K. Yuyama, T. Rungsimanon, T. Sugiyama, and H. Masuhara, Cryst. Growth Des., 12, 2427 (2012).
[4] K. Yuyama, C. S. Wu, T. Sugiyama, and H. Masuhara, Photochem. Photobiol. Sci., 13, 254 (2014).

Keywords: Optical Trapping, Crystallization, Protein