Probing stator dynamics and distributions of Escherichia coli flagellar motors by PALM
Tsai-Shun Lin1, Chien-Jung Lo1*
1Physics, National Central University, Taoyuan, Taiwan
* presenting author:Chien-Jung Lo, email:cjlo@phy.ncu.edu.tw
The bacterial flagellar motor is a natural electrical rotary molecular machine with a 45 nm basal body. The stator-unit consists of MotA/B proteins couples ion-flux to motor rotations. More than 10 stator-units in a motor turn over dynamically in response to the cellular energetical conditions and external loads. However, spatial features and temporal dynamics of stator-units have never been studied thoroughly. We have built a photoactivated localization microscopy (PALM) type super-resolution fluorescent microscope with 20 nm resolution to study stator-units dynamics using dual color fluorescent labeling on rotor- and stator- proteins. We will present stator protein counting, spatial distribution and dynamical properties in this talk.


Keywords: bacterial flagellar motor, stator, superresolution, PALM