Highly Sensitive Bio-sensing based on Fluorescence/Delayed Fluorescence Lifetime of Biocompatible Nano-materials
Maria Jessabel Aviles Talite1*, Chi-Tsu Yuan1
1Department of Physics, Chung Yuan Christian University, Chung Li, Taoyuan, Taiwan
* presenting author:Maria Jessabel Talite, email:jessatalite@gmail.com

Solution-processed gold nanoclusters (AuNCs) and carbon dots (CDs) exhibit excellent biocompatibility and unique luminescence properties that have been widely used as biological probes for bio-sensing and bio-imaging. Most of the aforementioned applications rely on the photoluminescence (PL) intensity, which suffers from some unavoidable drawbacks, in particular, in complex biological environment, such as concentration-induced PL variations. In contrast to conventional PL-intensity based bio-sensing, here the lifetime of fluorescence and thermally-activated delayed fluorescence (TADF) were used to sense the metal ions, oxygen concentration and biological molecules. We expect that high-performance bio-sensing can be achieved based on PL lifetime of AuNCs and CDs due to their relative longer lifetime as compared with that of most auto-fluorescence background.

Keywords: Gold Nanoclusters, Carbon dots, Thermally Activated Delayed Fluorescence, Bio-sensing, Metal ions